제품상세정보
DNA Polymerase
Uni Taq Polymerase
제품의 구성
| Products | Size | Contents | Cat. No |
|---|---|---|---|
| Uni Taq | 250 unit | 10X PCR buffer I | EP-001A |
| 10 X PCR buffer II | |||
| 500 unit | 10X PCR buffer I | EP-001B | |
| 10 X PCR buffer II | |||
| Uni Taq(with dNTPs) | 250 unit | 10X PCR buffer I | EPD-001A |
| 10 X PCR buffer II | |||
| 2.5 mM dNTPs | |||
| 500 unit | 10X PCR buffer I | EPD-001B | |
| 10 X PCR buffer II | |||
| 2.5 mM dNTPs |
Protocol
| General reaction mixture for PCR (100 ㎕) | ||
|---|---|---|
| Uni Taq ( 5 U/㎕) | 0.5 ㎕ | |
| 2.5 mM dNTPs | 8 ㎕ | |
| 10 X PCR buffer I, II or mix | 10 ㎕ | |
| Template DNA | 10 ~ 100 ng | |
| Primer 1 (10 pmole) | 1 ~ 2 ㎕ | |
| Primer 2 (10 pmole) | 1 ~ 2 ㎕ | |
| Sterilized deionized water | Up to 100 ㎕ | |
| General reaction cycle profile | ||
| 95°C | 3 min | 1 cycle |
| 94°C | 30 sec | 30 cycle |
| Annealing* | 30 sec | |
| 72°C | ~ 2 min* | |
| 72°C | 5 min | 1 cycle |
| *Annealing temperature : Tm - (~ 5 °C) / Tm : 2 X (A+T) + 4 X (G + C) | ||
| *Extension time ~ 2 kb : 1 min > 2 kb : 1 min + (2.5 sec / 100 bases) If PCR fragments are to be cloned into T/A vectors, the last extension step can be extended to up to 30 min. |
||
Experimental Data
